![]() ![]() The sample I used Nanodrop result is shown attached. And I am trying to use the reagent and primers concentration from a paper, and the amount of concentration for the primers and reagents I used is the same as the paper. Question about expanding the linear range for my standard curve dilution in Taqman probe qPCR? I am doing a 4-fold serial dilution using the Taqman probe assay to generate a standard curve dilution, and it was a multiplex qPCR. It is part of the SAGE University Press series "Quantitative Applications in the Social Sciences" ISBN 0-8039-4177-3. ![]() I also like "Multiple Comparison Procedures" by Larry E. If you just want to compare multiple comparison procedures I would suggest reading about them in the SAS documentation (you don't need to buy the program to read the documentation). A copy can be purchased through for about $70 US dollars. If you are mathematically inclined and have taken at least one class in statistics I might suggest: "Analysis of generalized linear mixed models in the agricultural and natural resources sciences" by Gbur et al.2012. The choice here is to spend several weeks/months with various textbooks that we could (collectively) suggest, or to talk to a statistician. There are also options like REML methods to avoid issues on non-normally distributed data. There are a large number of multiple comparison procedures. It would be well worth the effort to talk to a statistician at your university. There may also be critical features in your data and methods that we cannot see. There are many other options, especially if my guess is wrong. ![]()
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